To investigate the effects of diurnal alternation in natural environments on asphalt aging, an intermittent UV aging test was designed. The rheological properties of asphalt subjected to UV light-dark alternation aging were explored using a Dynamic Shear Rheometer (DSR), a Bending Beam Rheometer (BBR), and an Atomic Force Microscope (AFM). The results indicate that compared to continuous UV aging, the UV light-dark alternation has a more significant impact on the rheological properties of asphalt. After undergoing UV light-dark alternation aging, the high- and low-temperature rheological properties of the three types of base asphalts deteriorated more severely. Additionally, the asphalt's fluidity under medium temperature conditions decreased, and its temperature sensitivity was lower. At the same depth, the number and total area of the honeycomb structures in the asphalt decreased, while the aging substances diffused more thoroughly within the asphalt, leading to a greater degree of aging.