To study the rheological properties of heavy bio-oil recycled asphalt， the heavy bio-oil was mixed into 70^# asphalt and SBS modified asphalt aged by rotary film oven test （RTFOT） to prepare heavy bio-oil recycled asphalt， and the rheological properties were investigated via dynamic shear rheology （DSR） test. The results show that the fatigue resistance， low-temperature crack resistance and viscosity ratio of aged asphalt are improved and the high-temperature rutting resistance is reduced after mixing with heavy bio-oil. The asphalt is subject to irrecoverable permanent deformation at low frequency loading. The improvement effect of heavy bio-oil on 70^# aged asphalt is better than that of SBS modified asphalt. In addition， it is suggested that the suitable content of heavy bio-oil is 4%， because the properties of recycled asphalt are restored to the original asphalt at this content.