The SBS modified asphalt were prepared with three self-developed bio-based stabilizers （A，B，C）. The general technical properties of these asphalt were tested and compared. Then the high-temperature and low-temperature performances of asphalt were especially evaluated by the dynamic shear rheological（DSR） test and the bending beam rheological（BBR） test. Besides， the high-temperature rheological properties of asphalt were evaluated by the multiple stress creep recovery（MSCR） test， and the microscopic morphology of asphalt was analyzed by the fluorescence microscopy test. The cost of these bio-based stabilizers was also compared with that of traditional sulfur stabilizer. Results show that bio-based stabilizers can reduce the 135 ℃ viscosity of SBS modified asphalt by 12.6%-25.8% compared with commercial sulfur stabilizer； while stabilizer A and C can improve high-temperature performance and stabilizer A have a slight advantage in improving low-temperature performance. SBS modified asphalt prepared with stabilizer A has excellent high-temperature rheological properties and stabilizer C and sulfur stabilizer have their own advantages， while stabilizer B is not effective； stabilizer A can promote the dispersion of SBS in asphalt； the costs of bio-based stabilizers are similar to that of sulfur stabilizer.