In order to study the healing performance of asphalt under multiple damage conditions, the dynamic shear rheometer(DSR) time scanning mode was used to carry out damage healing cycle loading test. Recovery degree of complex modulus during healing was taken as healing index. Multiple damage healing properties of 70# matrix asphalt and SBS modified asphalt under different aging degrees were analyzed. Combined with Fourier infrared spectroscopy(FTIR), the influence of functional group composition and content on self healing ability was quantitatively analyzed. The results show that healing index decreased with the increase of the damage healing times. The healing index decreased the most when the first injury occurred. The degree of aging affected the healing index significantly. The higher the aging degree, the smaller the healing index. After multiple damage healing and aging, the healing performance of SBS modified asphalt is better than 70# matrix asphalt. SBS modifier can enhance the fatigue resistance and anti aging ability. The more macromolecular content, the slower the molecular diffusion rate and the self healing ability. The more lighter and longer molecular content, the faster the polymer diffusion rate, and the stronger the self healing ability.