Four point bending beam fatigue test(4PB), two point bending beam fatigue test(2PB), and overlay tester(OT) were used to evaluate the fatigue properties of seven kinds of asphalt mixtures that are usually used in engineering. The result shows that in three kinds of fatigue tests, the fatigue performances of 6%(by mass, the same below) SBS modified asphalt mixture is good, the fatigue performances of 10% TB+3% SBS modified asphalt mixture,45% SBS modified asphalt mixture and 45% SBS+04% polyphosphoric acid(PPA) modified asphalt mixture are fine, and the fatigue performance of 3%SBS modified asphalt mixture is inferior. The fatigue performances of warm mixing agent modified asphalt mixture and ESSO bitumen are the worst. The increase of SBS content can effectively improve the fatigue performance of the asphalt mixture. The fatigue performance of the SBS modified asphalt mixture can be improved by the addition of TB and PPA, but the effect on fatigue performance is not as big as that of increasing SBS content. In different tests, the ranking of fatigue performance of asphalt mixture is similar, and the specific ranking is slightly different.