When Sporosarcina pasteurii serves as bio healing agent of concrete, it is inevitably exposed to a complex environment. In order to study the bacteria activity and calcium carbonate production of Sporosarcina pasteurii in different conditions, experiments such as strong alkaline tolerance, urea concentration adaptability and temperature adaptability were conducted. Results show that the strong alkaline environment in concrete is close to, or even higher than, the limit of alkaline tolerance of the bacteria. Moreover, bacteria activity would be enhanced as urea concentration and temperature increase. It is recommended that the urea concentration in healing agent should be 06mol/L. Additionally, optimal application time of the healing agent is in summer. Results prove that the production of Sporosarcina pasteurii urea calcium acetate system is vaterite. This system can be used for concrete crack healing.