Nano adhesive force of SBS modified asphalt containing Sasobit and Evotherm 3G warm mix additives was determined by the force spectrum technique of atomic force microscopy. The nano adhesive force of SBS modified asphalt containing warm mix additive determined was verified based on surface free energy theory. And the nano adhesive force of SBS modified asphalt containing warm mix additives after immersion in water was also determined. Based on this, the change rate of the nano adhesive characteristics of the SBS modified asphalt containing warm mix additives was discussed. And the significant difference in the nano adhesive characteristics of the SBS modified asphalt containing warm mix additives was analyzed by mathematical statistics method. The results show that force spectroscopy technology of atomic force microscopy is an effective method to test directly the nano adhesive force. Although Sasobit slightly reduces nano adhesive force under dry condition, it can improve the nano adhesive force of asphalt in the presence of water. And the change rate of the nano adhesive force of the SBS modified asphalt containing Sasobit warm mix additive is lower before and after immersion in water. Evotherm 3G enhances nano adhesive force of SBS modified asphalt before and after immersion in water, and its change rate of the nano adhesive force is higher.