Influential factors and evaluation methods of healing behavior of asphalt binders were researched and developed based on dynamic shear rheometer tests with an intermittent loading sequence. Experiments were carried out to investigate fatigue behaviors of asphalt binder 70# and asphalt binder modified by SBS I C under 4 rest periods and 3strain levels. And the plateau values of the ratios of dissipated energy change in the experiments were calculated by dissipated energy theory. Through the analysis of the relationship between plateau values and rest periods, the healing rate(HR) was defined to quantify the healing potential of asphalt binders. The healing potentials were evaluated by HR and the healing index(HI). It is indicated that HI is improved with the increase of rest periods, HI and HR are decreased with the increase of strain levels, and both indexes of asphalt binder modified by SBS I C are superior to asphalt binder 70#.