Influence of disturbance period and vibration time on the mechanical properties of concrete was investigated by analyzing the compressive strength, flexural strength and damage degree. Results show that disturbance has the biggest influence on concrete strength when the penetration resistance is 107 to 148MPa. The maximum compressive strength loss reaches above 25% and the maximum flexural strength loss is more than 30%. The disturbance closer to initial setting and final setting has smaller effect on concrete and the average strength loss is about 10%. The extension of vibration time has little effect on the compressive strength of concrete. Nevertheless, its influence on the flexural strength is more significant. Flexural strength of concrete reduces more gently and the average flexural strength loss is about 10% when the vibration time is less than 30min. And when the vibration time is 40min, the average flexural strength loss is more than 30%. The damage of concrete increases with age significantly and the damage evolution value exceeds 5% when the penetration resistance is 107 to 148MPa and the vibration time is 30min. The damage of concrete shows no growth with age when the vibration time is 40min due to the large initial damage.