Based on the mass method, a simple and practical evaluation method of release amount of asphalt fume was put forward. The water interference to measurement accuracy of asphalt fume was eliminated by vacuum freeze drying technology. Pure polypropylene fiber cotton was preferred in five adsorption materials as superior performance adsorbent to concentrate asphalt fume. According to the principle of physical adsorption and characteristics of asphalt, a measurement equipment which combines generation device of asphalt fume, collection device of asphalt fume with emptier of asphalt fume was developed. The key factors which influence the release amount of asphalt fume were studied deeply at laboratory. It shows that mixing speed and the heating temperature and heating duration are the main factors that lead to the change of the release amount of asphalt fume; mixing or not， the different oil source and types of asphalt, the water content in asphalt all influence the release amount of asphalt fume.