Pullout tests of steel bar positioned in central of concrete specimen were conducted to investigation of the source rock state of sand, the content of stone powder and the concrete strength on the bond properties between deformed steel bar and concrete with machine made sand. The source rocks of sand were crushed stone and gravel, the contents of stone powder(by mass) were 5%, 9% and 13%, the strength grades of concrete were C30, C40, C50 and C60, the diameter of deformed steel bar was 12 mm. The results show that all specimens damaged from shear broken of concrete dentals in front of bar ribs and pullout of deformed bar, the bond slip curve can be represented as four phases by the characteristic values of bond stress and slip; the bond properties between deformed bar and concrete increase with the increasing concrete strength, which is better while the concrete mixed with sand made of crushed stone. The bond strength between deformed bar and concrete decreases with the increasing content of stone powder in machine made sand while the internal splitting phase of bond slip curve is prolonged and the decreasing phase becomes steeply. Based on the statistical analyses of test data, the constitutive relationships of bond slip between tensile deformed bar and concrete with machine made sand are proposed.