The chemical composition and revolution of warm mix rubber modified asphalt with EW and SDYK were analyzed by FTIR, and index of each functional group was calculated. The adhesion, DMT modulus and the revolution of nano-scale mechanical properties of rubber modified asphalt were analyzed by atomic force microscope (AFM) before and after thermo-oxidative aging. The results showed after the addition of EM and SDYK, there was no obvious chemical change in asphalt, but the content of asphalt components was changed.