In this paper, the lithology and alkali activity of low-grade metamorphic rocks in different areas of southeastern Guizhou Province were analyzed, the influence of total alkali contents on AAR was analyzed, and the optimum proportion of low-grade metamorphic rocks inhibition technology was investigated by orthogonal test. Results indicated that the saturated compressive strength of the low-grade metamorphic rocks was more discrete, and theses samples in each group were silicate rocks. The 14d expansion rate of the rock aggregates was more than 0.1%, indicating the potential harm from alkali aggregate reaction. The inhibition of fly ash on AAR was obvious, and the expansion rate of 14d in each group was not more than 0.03%. The greater the total alkali content of concretes, the faster the alkali-aggregate reaction, the longer the reaction period and the higher the swelling rate. The alkali content should be limited in 1%. From SEM-EDS, comparing the specimen without AAR, it was seen that a honeycomb-like alkali-silicic acid gel product was formed on the aggregate surface, the aggregate surface had fine cracks, and the Si and Na elements were enriched in the specimen in which the alkali aggregate reaction occurred. According to the environment, structural and requirements of concretes with low-grade metamorphic rock aggregates, it is necessary to optimize the mineral admixture or inhibitor and strictly control the alkali content. It is also very important to observe and test the effect of AAR on the concrete with low-grade metamorphic rocks aggregates for a long time.