Six 3D printing structural columns and three common reinforced concrete columns were tested under eccentric loading. The failure characteristics, deflection, and bearing capacity of 3D printing structural columns and common reinforced concrete columns were compared and analyzed. Experimental results indicate that failure mode of 3D printing structural column is similar to common reinforced concrete column, the bearing capacity of 3D printing structural column decreases by 10%20%, and with the increase of eccentricity, the growth of maximum deflection is also increased.